Zeiss LSM710 NLO 2-photon
Intracellular physiology can be dependably studied in intact organisms only. By multiphoton microscopy it is possible to explore fundamental mechanisms in functional tissue. A perfect choice for neurobiologists, developmental biologists, immunologists and plant biologists: the 2-photon microscope captures high-resolution images of fluorescent structures in living animals and thick tissue specimen. Our new 2-photon microscope (Zeiss 710 NLO) has a high signal-to-noise ratio enabling brilliant imaging even in deep-lying tissue layers.
Pulsed, ultrafast infrared lasers excite fluorescent dyes at the focus only – the only place where photon density is high enough to produce fluorescence. With low absorption and next to no scattering loss, the red excitation light penetrates much deeper into tissue. With some tissues it excites fluorochromes at depths up to 1 mm. Thanks to the small excitation volume, images of subcellular resolution are obtained. Being only excited at the focal plane, the dye won’t bleach above and below it: ensuring unmatched preservation of your fluorescence-labeled specimen.
- Femtosecond biphoton laser (690-1040 nm) plus single-photon excitation lasers (405, 458, 476, 488, 514, 543 and 633 nm). The LSM710 can also be used as a traditional confocal microscope with its 3 descanned detectors.
- Non-descanned detectors with 3 combinations of emission filters (blue⁄green, green⁄red, CFP⁄YFP). Fluorescence light excited with the multiphoton laser originates from the focus only. Thus, no confocal pinhole is needed, and the signal can be projected directly from the specimen onto the detector, gaining sensitivity.
- GaAsP non-descanned detector. High efficiency detection (up to twice the level of that of standard non-descanned detectors) and low dark noise: the ideal tool for visualizing very fine details in tissue that may be extremely light-scattering.
- The system is equipped with a very high NA, long distance, 20x dip-in objective, a 40x dip-in and a 63x oil objective.