For cell biology the use of luminescence bears the advantages of stronger sensitivity and highest signal to noise ratio compared to fluorescence. With conventional luminometers it was so far only possible to measure the average luminescence intensity of a sample, but particularly for the analysis of cultured cells or tissues it is of great interest for many researchers to resolve cellular structures in detail.
At the Bioimaging Platform we have now installed a system that is able to just do this.
The LV200 has been developed by Olympus and could be best described as an imaging luminometer. Based on a new optical concept and special components the LV200 allows not only to characterize the specimen by the luminescence intensity but to image cellular samples and to quantify the luminescence structures at cellular and even sub-cellular resolution.
The imaging luminometer is therefore ideally suited for the long-term observation of luminescence based reporter gene assays where phototoxicity of the fluorescence excitation is an issue.