The Leica TCS SP5 gatedSTED CW is a super-resolution confocal microscope.

Stimulated Emission Depletion (STED) is a special illumination technique allowing the resolution of sub-cellular details below 80 nm. For STED imaging, a Continuous Wave (CW) laser enables the use of conventional dyes such as Alexa 488, FITC and Oregon Green and established fluorescent proteins such as YFP. HyD detectors combined with this laser enables time gating, thus further increasing resolution to below 50nm.


The microscope features 3 classical PMTs, a transmission PMT, plus:

> 2 HyD detectors with increased sensitivity, allowing the capture of weaker signals or faster imaging.
> 2 Avalanche Photodiodes (APD) for very low signal imaging.

  • Beside a classical line scanner, a resonance scanner is also present for faster imaging.
  • 456, 488, 514, 561, 633 nm laserlines are also present for traditional confocal imaging + a White Light laser (470-680nm, tunable). Note that the 405 nm laser is not present because its slot is occupied by the CW laser. DAPI should be replaced by DRAQ5 whenever a nuclear staining is needed.
  • Multi-positions, tile scanning, bleaching, etc.